SB52
(Plasmid
#21487)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21487 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA3
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCited2
-
Alt namep35srj (MRG1)
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1200
-
GenBank IDAF109161
-
Entrez GeneCITED2 (a.k.a. ASD8, MRG-1, MRG1, P35SRJ, VSD2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ecoRI (not destroyed)
- 3′ cloning site rsaI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ligated into the EcoRI/EcoRV sites of pcDNA3
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
SB52 was a gift from Shoumo Bhattacharya (Addgene plasmid # 21487 ; http://n2t.net/addgene:21487 ; RRID:Addgene_21487) -
For your References section:
Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1. Bhattacharya S, Michels CL, Leung MK, Arany ZP, Kung AL, Livingston DM. Genes Dev. 1999 Jan 1. 13(1):64-75. 10.1101/gad.13.1.64 PubMed 9887100