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Addgene

ATM_N_FS_v2_Donor
(Plasmid #214815)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214815 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2364
  • Total vector size (bp) 4250
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3xFLAG_2xStrep for tagging ATM at the N-terminus
  • Alt name
    ATM
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    885
  • GenBank ID
  • Entrez Gene
    ATM (a.k.a. AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1)
  • Tag / Fusion Protein
    • 3xFLAG_2xStrep (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Donor plasmid for tagging ATM at its N-terminus. Use in combination with U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2 (Addgene plasmid # 214814).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ATM_N_FS_v2_Donor was a gift from Yannick Doyon (Addgene plasmid # 214815 ; http://n2t.net/addgene:214815 ; RRID:Addgene_214815)
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