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Addgene

pET-28a(+)-T7-OmpA-MNaseB-6xHis
(Plasmid #214809)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214809 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5242
  • Total vector size (bp) 5809
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    OmpA-Micrococcal nuclease B
  • Alt name
    OmpA-MNaseB
  • Species
    Staphylococcus aureus
  • Insert Size (bp)
    567
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • 6xhis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28a(+)-T7-OmpA-MNaseB-6xHis was a gift from Peter Sarin (Addgene plasmid # 214809 ; http://n2t.net/addgene:214809 ; RRID:Addgene_214809)

  • For your References section:

    Purification of micrococcal nuclease for use in ribosomal profiling of high-salinity extremophiles. Gregorova P, Isada M, DiRuggiero J, Sarin LP. JBC, 108020, Nov 2024 10.1016/j.jbc.2024.108020
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