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pMSCV-IRES-mOrange/CALR del52
(Plasmid #214768)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214768 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV-IRES-mOrange
  • Backbone size w/o insert (bp) 6500
  • Modifications to backbone
    modify pMSCV-IRES-GFP(replace GFP to mOrange)
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human CALR del52
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5496
  • Entrez Gene
    CALR (a.k.a. CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR)
  • Promoter MSCV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer original
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-IRES-mOrange/CALR del52 was a gift from Norio Komatsu (Addgene plasmid # 214768 ; http://n2t.net/addgene:214768 ; RRID:Addgene_214768)

  • For your References section:

    Clinical and biological relevance of CREB3L1 in Philadelphia chromosome-negative myeloproliferative neoplasms. De Marchi F, Okuda M, Morishita S, Imai M, Baba T, Horino M, Mori Y, Furuya C, Ogata S, Yang Y, Ando J, Ando M, Araki M, Komatsu N. Leuk Res. 2022 Aug;119:106883. doi: 10.1016/j.leukres.2022.106883. Epub 2022 May 31. 10.1016/j.leukres.2022.106883 PubMed 35689957
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