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Addgene

pRSFDuet-gst-xr
(Plasmid #214761)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214761 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3829
  • Total vector size (bp) 5485
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    glutathione S-transferases fused with xylose reductase
  • Alt name
    gst-xyl
  • Alt name
    gst-xr
  • Species
    Hypocrea jecorina
  • Insert Size (bp)
    1658
  • Promoter T7
  • Tag / Fusion Protein
    • Glutathione S-transferases (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTATAAGAAGGAGATATACAATGTCCCCTATACTAGGTTATTG
  • 3′ sequencing primer TATCCAATTGAGATCTGCCAAATTCTTAACCAAACAGGTAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSFDuet-gst-xr was a gift from Pimchai Chaiyen (Addgene plasmid # 214761 ; http://n2t.net/addgene:214761 ; RRID:Addgene_214761)

  • For your References section:

    A versatile in situ cofactor enhancing system for meeting cellular demands for engineered metabolic pathways. Jaroensuk J, Sutthaphirom C, Phonbuppha J, Chinantuya W, Kesornpun C, Akeratchatapan N, Kittipanukul N, Phatinuwat K, Atichartpongkul S, Fuangthong M, Pongtharangkul T, Hollmann F, Chaiyen P. J Biol Chem. 2023 Dec 28:105598. doi: 10.1016/j.jbc.2023.105598. 10.1016/j.jbc.2023.105598 PubMed 38159859
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