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Addgene

pBTK522-FAST-PETase
(Plasmid #214754)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214754 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBTK522
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For expression in P. putida
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FAST-PETase

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBTK522-FAST-PETase was a gift from Hal Alper (Addgene plasmid # 214754 ; http://n2t.net/addgene:214754 ; RRID:Addgene_214754)

  • For your References section:

    Machine learning-aided engineering of hydrolases for PET depolymerization. Lu H, Diaz DJ, Czarnecki NJ, Zhu C, Kim W, Shroff R, Acosta DJ, Alexander BR, Cole HO, Zhang Y, Lynd NA, Ellington AD, Alper HS. Nature. 2022 Apr;604(7907):662-667. doi: 10.1038/s41586-022-04599-z. Epub 2022 Apr 27. 10.1038/s41586-022-04599-z PubMed 35478237
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