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Addgene

pMBA323
(Plasmid #214751)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214751 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    oriR101
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
  • Alt name
    p19-RBS(5392)-nadE + p19-sacB
  • Species
    other

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMBA323 was a gift from Tae Seok Moon (Addgene plasmid # 214751 ; http://n2t.net/addgene:214751 ; RRID:Addgene_214751)

  • For your References section:

    Engineering E. coli strains using antibiotic-resistance-gene-free plasmids. Amrofell MB, Rengarajan S, Vo ST, Ramirez Tovar ES, LoBello L, Dantas G, Moon TS. Cell Rep Methods. 2023 Dec 18;3(12):100669. doi: 10.1016/j.crmeth.2023.100669. Epub 2023 Dec 11. 10.1016/j.crmeth.2023.100669 PubMed 38086386
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