pMBA332
(Plasmid
#214744)
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PurposeLow copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214744 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSC101
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Vector typeBacterial Expression
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Selectable markersAuxotrophic-based plasmid selection
Growth in Bacteria
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Bacterial Resistance(s)None
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Growth Temperature37°C
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Growth Strain(s)DH10B ΔauxG
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameBBa_J23119-RiboJ-RBS(21992)-gfpmut3
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Alt nameBBa_J23108-RBS(7504)-thyA-RBS(4427)-infA
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Speciesother
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains three mutations in Rep101. These mutations are not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMBA332 was a gift from Tae Seok Moon (Addgene plasmid # 214744 ; http://n2t.net/addgene:214744 ; RRID:Addgene_214744) -
For your References section:
Engineering E. coli strains using antibiotic-resistance-gene-free plasmids. Amrofell MB, Rengarajan S, Vo ST, Ramirez Tovar ES, LoBello L, Dantas G, Moon TS. Cell Rep Methods. 2023 Dec 18;3(12):100669. doi: 10.1016/j.crmeth.2023.100669. Epub 2023 Dec 11. 10.1016/j.crmeth.2023.100669 PubMed 38086386
