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Addgene

pLenti CMV V5-LUC Blast (w567-1) Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: ClaI and SacII (double digest).

We expected the following band sizes for this digest: 3kb & 6.5kb. We see the expected pattern, accounting for some single cut plasmid running at approx. 9.5kb.