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Addgene

AAVS1-eTLR
(Plasmid #214737)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214737 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBlueScript
  • Backbone size w/o insert (bp) 3948
  • Total vector size (bp) 11147
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AAVS1-eTLR
  • Species
    H. sapiens (human), G. gallus (chicken), B. taurus (bovine), O. cuniculus (rabbit), Synthetic; CMV, Anguilla japonica, influenza
  • Insert Size (bp)
    7199
  • Entrez Gene
    PPP1R12C (a.k.a. AAVS1, LENG3, MBS85, p84, p85)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1-eTLR was a gift from Gil Westmeyer (Addgene plasmid # 214737 ; http://n2t.net/addgene:214737 ; RRID:Addgene_214737)
  • For your References section:

    Exonuclease-enhanced prime editors. Truong DJ, Geilenkeuser J, Wendel SV, Wilming JCH, Armbrust N, Binder EMH, Santl TH, Siebenhaar A, Gruber C, Phlairaharn T, Zivanic M, Westmeyer GG. Nat Methods. 2024 Feb 1. doi: 10.1038/s41592-023-02162-w. 10.1038/s41592-023-02162-w PubMed 38302659