AAVS1-eTLR
(Plasmid
#214737)
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Purposeencodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214737 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBlueScript
- Backbone size w/o insert (bp) 3948
- Total vector size (bp) 11147
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Vector typeMammalian Expression, CRISPR, Synthetic Biology
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAAVS1-eTLR
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SpeciesH. sapiens (human), G. gallus (chicken), B. taurus (bovine), O. cuniculus (rabbit), Synthetic; CMV, Anguilla japonica, influenza
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Insert Size (bp)7199
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Entrez GenePPP1R12C (a.k.a. AAVS1, LENG3, MBS85, p84, p85)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAVS1-eTLR was a gift from Gil Westmeyer (Addgene plasmid # 214737 ; http://n2t.net/addgene:214737 ; RRID:Addgene_214737) -
For your References section:
Exonuclease-enhanced prime editors. Truong DJ, Geilenkeuser J, Wendel SV, Wilming JCH, Armbrust N, Binder EMH, Santl TH, Siebenhaar A, Gruber C, Phlairaharn T, Zivanic M, Westmeyer GG. Nat Methods. 2024 Feb 1. doi: 10.1038/s41592-023-02162-w. 10.1038/s41592-023-02162-w PubMed 38302659