pCAG_iPE-C
(Plasmid
#214735)
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Purposeencodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214735 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBlueScript
- Backbone size w/o insert (bp) 2825
- Total vector size (bp) 11257
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Vector typeMammalian Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCAG_iPE-C_bGH
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SpeciesH. sapiens (human), G. gallus (chicken), B. taurus (bovine), O. cuniculus (rabbit), Synthetic; S.pyogenes, MMLV, CMV, SV40, influenza
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Insert Size (bp)8435
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG_iPE-C was a gift from Gil Westmeyer (Addgene plasmid # 214735 ; http://n2t.net/addgene:214735 ; RRID:Addgene_214735) -
For your References section:
Exonuclease-enhanced prime editors. Truong DJ, Geilenkeuser J, Wendel SV, Wilming JCH, Armbrust N, Binder EMH, Santl TH, Siebenhaar A, Gruber C, Phlairaharn T, Zivanic M, Westmeyer GG. Nat Methods. 2024 Feb 1. doi: 10.1038/s41592-023-02162-w. 10.1038/s41592-023-02162-w PubMed 38302659
