SpyTag-Quartet [SARS1]
(Plasmid
#214729)
-
PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV each separated by GS-linkers with an N-terminal SpyTag fusion
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 214729 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3.1
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5568
- Total vector size (bp) 8397
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSpyTag-Quartet [SARS1]
-
SpeciesSynthetic
-
Insert Size (bp)2828
-
Entrez GeneS (a.k.a. sars2, E2)
- Promoter CMV
-
Tag
/ Fusion Protein
- SpyTag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
SpyTag-Quartet [SARS1] was a gift from Mark Howarth (Addgene plasmid # 214729 ; http://n2t.net/addgene:214729 ; RRID:Addgene_214729) -
For your References section:
Proactive vaccination using multiviral Quartet Nanocages to elicit broad anti-coronavirus responses. Hills RA, Tan TK, Cohen AA, Keeffe JR, Keeble AH, Gnanapragasam PNP, Storm KN, Rorick AV, West AP Jr, Hill ML, Liu S, Gilbert-Jaramillo J, Afzal M, Napier A, Admans G, James WS, Bjorkman PJ, Townsend AR, Howarth MR. Nat Nanotechnol. 2024 May 6. doi: 10.1038/s41565-024-01655-9. 10.1038/s41565-024-01655-9 PubMed 38710880