pLenti PGK V5-LUC Neo (w623-2)
(Plasmid #21471)

• Purpose: 3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Neo
• Depositing Lab: Eric Campeau
• Publication: Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size w/o insert (bp): 7334
• Vector type: Mammalian Expression, Lentiviral, Luciferase
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3, 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: V5-LUC
• Alt name: Firefly Luciferase
• Species: Firefly
• Insert Size (bp): 1776
• Tag / Fusion Protein:
  • V5 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bam HI (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: PGKforw
• 3′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Firefly luciferase cDNA derived from the pGL3 plasmid.
• Articles Citing this Plasmid:
  • 29 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry