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Addgene

pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAG
(Plasmid #214706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV-IRES-GFP
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CALR ins5 YD/FL-FLAG
  • Species
    H. sapiens (human)
  • Entrez Gene
    CALR (a.k.a. CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR)
  • Promoter MSCV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAG was a gift from Norio Komatsu (Addgene plasmid # 214706 ; http://n2t.net/addgene:214706 ; RRID:Addgene_214706)

  • For your References section:

    Mutant calreticulin interacts with MPL in the secretion pathway for activation on the cell surface. Masubuchi N, Araki M, Yang Y, Hayashi E, Imai M, Edahiro Y, Hironaka Y, Mizukami Y, Kihara Y, Takei H, Nudejima M, Koike M, Ohsaka A, Komatsu N. Leukemia. 2020 Feb;34(2):499-509. doi: 10.1038/s41375-019-0564-z. Epub 2019 Aug 28. 10.1038/s41375-019-0564-z PubMed 31462733
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