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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21470 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBSKS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2900
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Vector typeCloning Vector
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Selectable markersN/A
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNotch4
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SpeciesM. musculus (mouse)
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Insert Size (bp)6677
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GenBank IDU43691
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Entrez GeneNotch4 (a.k.a. Int-3, Int3, N4)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS Notch4HA was a gift from Jan Kitajewski (Addgene plasmid # 21470 ; http://n2t.net/addgene:21470 ; RRID:Addgene_21470) -
For your References section:
Notch4 and Wnt-1 proteins function to regulate branching morphogenesis of mammary epithelial cells in an opposing fashion. Uyttendaele H, Soriano JV, Montesano R, Kitajewski J. Dev Biol. 1998 Apr 15. 196(2):204-17. 10.1006/dbio.1998.8863 PubMed 9576833