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Addgene

pMSCV-IRES-GFP/CALR wt
(Plasmid #214699)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214699 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV-IRES-GFP
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CALR wt
  • Species
    H. sapiens (human)
  • Entrez Gene
    CALR (a.k.a. CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR)
  • Promoter MSCV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer MSCV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-IRES-GFP/CALR wt was a gift from Norio Komatsu (Addgene plasmid # 214699 ; http://n2t.net/addgene:214699 ; RRID:Addgene_214699)

  • For your References section:

    Mutant calreticulin interacts with MPL in the secretion pathway for activation on the cell surface. Masubuchi N, Araki M, Yang Y, Hayashi E, Imai M, Edahiro Y, Hironaka Y, Mizukami Y, Kihara Y, Takei H, Nudejima M, Koike M, Ohsaka A, Komatsu N. Leukemia. 2020 Feb;34(2):499-509. doi: 10.1038/s41375-019-0564-z. Epub 2019 Aug 28. 10.1038/s41375-019-0564-z PubMed 31462733
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