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Addgene

FLAG NC
(Plasmid #214675)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214675 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    E2.pEF5-DEST-FL-V5-MCP
  • Backbone manufacturer
    Modified from ThermoFisher
  • Backbone size w/o insert (bp) 7357
  • Modifications to backbone
    MS2 Coat Protein Added to C-terminal
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG
  • Species
    Synthetic
  • Insert Size (bp)
    180
  • Promoter EF-1a
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • MCP (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer Full plasmid sequencing used
  • 3′ sequencing primer Full plasmid sequencing used
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG NC was a gift from Eugene Yeo (Addgene plasmid # 214675 ; http://n2t.net/addgene:214675 ; RRID:Addgene_214675)

  • For your References section:

    Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion. Schmok JC, Jain M, Street LA, Tankka AT, Schafer D, Her HL, Elmsaouri S, Gosztyla ML, Boyle EA, Jagannatha P, Luo EC, Kwon EJ, Jovanovic M, Yeo GW. Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-02014-0. 10.1038/s41587-023-02014-0 PubMed 38168984
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