lucMAPT-GenRep
(Plasmid #214674)

• Purpose: MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exons
• Depositing Lab: Eugene Yeo
• Publication: Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-02014-0.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5420
• Total vector size (bp): 10202
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MAPT
• Species: H. sapiens (human)
• Insert Size (bp): 4782
• Mutation: Contains Exon 9 and Exon 11, with a BamHI EcoRI cloning site in the middle, and ~900bp intronic sequences separating the cloning site from the exons
• Entrez Gene: MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Firefly Luciferase (N terminal on insert)
  • Renilla Luciferase (C terminal on insert)
  • V5 (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Full-plasmid sequencing used
• 3′ sequencing primer: Full-plasmid sequencing used

Resource Information

• Supplemental Documents:
  • GenRep_03.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry