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Addgene

lucMAPT-GenRep
(Plasmid #214674)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214674 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5420
  • Total vector size (bp) 10202
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAPT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4782
  • Mutation
    Contains Exon 9 and Exon 11, with a BamHI EcoRI cloning site in the middle, and ~900bp intronic sequences separating the cloning site from the exons
  • Entrez Gene
    MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Firefly Luciferase (N terminal on insert)
    • Renilla Luciferase (C terminal on insert)
    • V5 (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer Full-plasmid sequencing used
  • 3′ sequencing primer Full-plasmid sequencing used
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lucMAPT-GenRep was a gift from Eugene Yeo (Addgene plasmid # 214674 ; http://n2t.net/addgene:214674 ; RRID:Addgene_214674)

  • For your References section:

    Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion. Schmok JC, Jain M, Street LA, Tankka AT, Schafer D, Her HL, Elmsaouri S, Gosztyla ML, Boyle EA, Jagannatha P, Luo EC, Kwon EJ, Jovanovic M, Yeo GW. Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-02014-0. 10.1038/s41587-023-02014-0 PubMed 38168984
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