pTag4C_SP-HTR2A-V2R-NTEV-tcs-GV-2xHA
(Plasmid
#214615)
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PurposeHTR2A receptor for split TEV assay
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214615 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTag4C
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHTR2A-NTEV
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SpeciesH. sapiens (human)
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Entrez GeneHTR2A (a.k.a. 5-HT2A, HTR2)
- Promoter CMV
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Tag
/ Fusion Protein
- TEV-Gal4-VP16-2xHA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTag4C_SP-HTR2A-V2R-NTEV-tcs-GV-2xHA was a gift from Michael Wehr (Addgene plasmid # 214615 ; http://n2t.net/addgene:214615 ; RRID:Addgene_214615) -
For your References section:
Protocol for identifying properties of ERBB receptor antagonists using the barcoded ERBBprofiler assay. Popovic L, Rossner MJ, Wehr MC. STAR Protoc. 2024 Apr 16;5(2):102987. doi: 10.1016/j.xpro.2024.102987. 10.1016/j.xpro.2024.102987 PubMed 38635397