pcDNA3_ERBB2-V5
(Plasmid
#214612)
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PurposeERBB2 receptor for ERBB2/ERBB3 heterodimer split TEV recuitment assay
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214612 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameERBB2
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SpeciesH. sapiens (human)
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Entrez GeneERBB2 (a.k.a. CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2))
- Promoter CMV
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3_ERBB2-V5 was a gift from Michael Wehr (Addgene plasmid # 214612 ; http://n2t.net/addgene:214612 ; RRID:Addgene_214612) -
For your References section:
Protocol for identifying properties of ERBB receptor antagonists using the barcoded ERBBprofiler assay. Popovic L, Rossner MJ, Wehr MC. STAR Protoc. 2024 Apr 16;5(2):102987. doi: 10.1016/j.xpro.2024.102987. 10.1016/j.xpro.2024.102987 PubMed 38635397