pMIA10.7
(Plasmid #214451)

• Purpose: BxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.
• Depositing Lab: Roger Foo
• Publication: Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.7554/eLife.79592.

Backbone

• Vector backbone: pMIA10.5-Clover
• Backbone manufacturer: in house
• Vector type: BxbI donor
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Tet-On 3G
• Species: Synthetic
• Insert Size (bp): 744
• Tags / Fusion Proteins:
  • T2A (C terminal on insert)
  • PuroR (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: PuroR
• Species: Synthetic
• Insert Size (bp): 600
• Tags / Fusion Proteins:
  • T2A (N terminal on insert)
  • Tet-On 3G (N terminal on insert)

Gene/Insert 3

• Gene/Insert name: TRE3G CopGFP
• Species: Synthetic
• Insert Size (bp): 1075

Resource Information

• Supplemental Documents:
  • pMIA10.7.gb
• A portion of this plasmid was derived from a plasmid made by: TRE: pTRE3G (Clontech, 631173) copGFP-SV40pA: pMAX-GFP (Lonza) Tet-On: pCMV-Tet-On 3G (Clontech, 631335)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry