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Addgene

pMIA3-Pansio
(Plasmid #214445)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214445 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIA3
  • Backbone size w/o insert (bp) 9825
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eSpCas9-P2A-mRuby2-P2A-tp53dn
  • gRNA/shRNA sequence
    Pansio-1 GSH targeting gRNA (430rev)
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIA3-Pansio was a gift from Roger Foo (Addgene plasmid # 214445 ; http://n2t.net/addgene:214445 ; RRID:Addgene_214445)

  • For your References section:

    Computationally defined and in vitro validated putative genomic safe harbour loci for transgene expression in human cells. Autio MI, Motakis E, Perrin A, Bin Amin T, Tiang Z, Do DV, Wang J, Tan JKM, Ding SSL, Tan WX, Lee CJM, Teo AKK, Foo R. Elife. 2024 Jan 2;13:e79592. doi: 10.7554/eLife.79592. 10.7554/eLife.79592 PubMed 38164941
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