8GCY
(Plasmid
#214440)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214440 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepET28-MHL (Addgene #26096)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCBLB:PBC020-G02:C248501
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Alt nameCBLB
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Alt nameCBLB_3Mut
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SpeciesH. sapiens (human)
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Insert Size (bp)1170
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Entrez GeneCBLB (a.k.a. ADMIO3, Cbl-b, Nbla00127, RNF56)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis-TEV (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PBD link: https://www.rcsb.org/structure/8GCY
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
8GCY was a gift from Cheryl Arrowsmith (Addgene plasmid # 214440 ; http://n2t.net/addgene:214440 ; RRID:Addgene_214440)