8DYS
(Plasmid
#214439)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214439 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepFBOH-MHL (Addgene #62304)
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Vector typeBaculovirus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEIF2A:PBC022-C06:C245159
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Alt nameEIF2A
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Alt nameEIF2A:1-585_BV
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SpeciesH. sapiens (human)
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Insert Size (bp)1755
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Entrez GeneEIF2A (a.k.a. CDA02, EIF-2A, MST089, MSTP004, MSTP089)
- Promoter Polyhedrin
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Tag
/ Fusion Protein
- 6xHis-TEV (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
- 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3') (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PBD link: https://www.rcsb.org/structure/8DYS
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
8DYS was a gift from Cheryl Arrowsmith (Addgene plasmid # 214439 ; http://n2t.net/addgene:214439 ; RRID:Addgene_214439)