PMP34-mApple-NFAST(low)
(Plasmid
#214418)
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PurposePeroxisome localization of low-affinity NFAST fused to PMP34-Apple
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214418 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePMP34-mApple-NFAST
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRspA-N
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SpeciesOther
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Entrez GeneSLC25A17 (a.k.a. PMP34)
- Promoter CMV
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Tag
/ Fusion Protein
- mApple (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn2I (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PMP34-mApple-NFAST(low) was a gift from Chi-Lun Chang (Addgene plasmid # 214418 ; http://n2t.net/addgene:214418 ; RRID:Addgene_214418) -
For your References section:
A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction. Li X, Gamuyao R, Wu ML, Cho WJ, Kurtz NB, King SV, Petersen RA, Stabley DR, Lindow C, Climer L, Shirinifard A, Ferrara F, Throm RE, Robinson CG, Carisey A, Tebo AG, Chang CL. bioRxiv. 2023 Nov 29:2023.11.29.569289. doi: 10.1101/2023.11.29.569289. Preprint. 10.1101/2023.11.29.569289 PubMed 38076863