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Addgene

Halotag-6xHp
(Plasmid #214414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Halo-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    6x Spastin aa 43-92
  • Species
    H. sapiens (human)
  • Entrez Gene
    SPAST (a.k.a. ADPSP, FSP2, SPG4)
  • Promoter CMV
  • Tag / Fusion Protein
    • Halo (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BseGI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Halotag-6xHp was a gift from Chi-Lun Chang (Addgene plasmid # 214414 ; http://n2t.net/addgene:214414 ; RRID:Addgene_214414)

  • For your References section:

    A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction. Li X, Gamuyao R, Wu ML, Cho WJ, King SV, Petersen RA, Stabley DR, Lindow C, Climer LK, Shirinifard A, Ferrara F, Throm RE, Robinson CG, Zhou Y, Carisey AF, Tebo AG, Chang CL. J Cell Biol. 2024 Sep 2;223(9):e202311126. doi: 10.1083/jcb.202311126. Epub 2024 Jul 1. 10.1083/jcb.202311126 PubMed 38949658
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