Halotag-6xHp
(Plasmid
#214414)
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PurposeLipid droplet localization of 6xHp of Spastin fused to Halotag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214414 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneHalo-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name6x Spastin aa 43-92
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SpeciesH. sapiens (human)
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Entrez GeneSPAST (a.k.a. ADPSP, FSP2, SPG4)
- Promoter CMV
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Tag
/ Fusion Protein
- Halo (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BseGI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Halotag-6xHp was a gift from Chi-Lun Chang (Addgene plasmid # 214414 ; http://n2t.net/addgene:214414 ; RRID:Addgene_214414) -
For your References section:
A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction. Li X, Gamuyao R, Wu ML, Cho WJ, King SV, Petersen RA, Stabley DR, Lindow C, Climer LK, Shirinifard A, Ferrara F, Throm RE, Robinson CG, Zhou Y, Carisey AF, Tebo AG, Chang CL. J Cell Biol. 2024 Sep 2;223(9):e202311126. doi: 10.1083/jcb.202311126. Epub 2024 Jul 1. 10.1083/jcb.202311126 PubMed 38949658
