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Addgene

pCMV-SlugCBE-NNG-P2A-puro
(Plasmid #214355)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214355 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SlugCBE-NNG and puromycin resistance genes
  • Species
    Synthetic
  • Mutation
    Q782R/S888R/L906R/N984S/E1012K/K1016I

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-SlugCBE-NNG-P2A-puro was a gift from Yongming Wang (Addgene plasmid # 214355 ; http://n2t.net/addgene:214355 ; RRID:Addgene_214355)

  • For your References section:

    Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM. Qi T, Wang Y, Yang Y, Gao S, Liu J, Huang Q, Tian Y, Tang J, Zheng WV, Wang Y. Nat Chem Biol. 2023 Dec 5. doi: 10.1038/s41589-023-01481-5. 10.1038/s41589-023-01481-5 PubMed 38052959
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