pSK-Sasg
(Plasmid
#214350)
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PurposeExpresses cloning backbone for SlugCas9 sgRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214350 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePUC57
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSlugCas9 tracrRNA
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSK-Sasg was a gift from Yongming Wang (Addgene plasmid # 214350 ; http://n2t.net/addgene:214350 ; RRID:Addgene_214350) -
For your References section:
Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM. Qi T, Wang Y, Yang Y, Gao S, Liu J, Huang Q, Tian Y, Tang J, Zheng WV, Wang Y. Nat Chem Biol. 2023 Dec 5. doi: 10.1038/s41589-023-01481-5. 10.1038/s41589-023-01481-5 PubMed 38052959