MP6-SlugABEN-738
(Plasmid
#214348)
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PurposeExpresses N-terminus of ABE, and untargeted Bacterial Mutagenesis
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214348 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCloDF13
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namednaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE
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Speciesother
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MP6-SlugABEN-738 was a gift from Yongming Wang (Addgene plasmid # 214348 ; http://n2t.net/addgene:214348 ; RRID:Addgene_214348) -
For your References section:
Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM. Qi T, Wang Y, Yang Y, Gao S, Liu J, Huang Q, Tian Y, Tang J, Zheng WV, Wang Y. Nat Chem Biol. 2023 Dec 5. doi: 10.1038/s41589-023-01481-5. 10.1038/s41589-023-01481-5 PubMed 38052959