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pET28a_Caspase-4_lemur_catalytic domain
(Plasmid #214328)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214328 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CASP4
  • Alt name
    Caspase-4 enzymatic domain
  • Species
    Microcebus murinus (mouse lemur)
  • Promoter T7
  • Tag / Fusion Protein
    • His-TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7-fwd
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a_Caspase-4_lemur_catalytic domain was a gift from Jonathan Kagan (Addgene plasmid # 214328 ; http://n2t.net/addgene:214328 ; RRID:Addgene_214328)

  • For your References section:

    Structural insights into cytokine cleavage by inflammatory caspase-4. Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC. Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9. Epub 2023 Nov 22. 10.1038/s41586-023-06751-9 PubMed 37993712
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