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Addgene

pMSCV-human-pro-IL-18_E192K_D193K
(Plasmid #214318)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214318 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV-IRES-EGFP
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IL18
  • Alt name
    pro-IL-18
  • Alt name
    Pro-Interleukin-18
  • Species
    H. sapiens (human)
  • Mutation
    E192K_D193K
  • Entrez Gene
    IL18 (a.k.a. IGIF, IL-18, IL-1g, IL1F4)
  • Promoter MESV
  • Tags / Fusion Proteins
    • Myc (N terminal on insert)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer MSCV-fwd
  • 3′ sequencing primer MSCV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-human-pro-IL-18_E192K_D193K was a gift from Jonathan Kagan (Addgene plasmid # 214318 ; http://n2t.net/addgene:214318 ; RRID:Addgene_214318)

  • For your References section:

    Structural insights into cytokine cleavage by inflammatory caspase-4. Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC. Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9. Epub 2023 Nov 22. 10.1038/s41586-023-06751-9 PubMed 37993712
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