pMSCV-human-pro-IL-18
(Plasmid
#214317)
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PurposeExpression of gene in mammalian cells by retroviral transduction
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214317 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMSCV-IRES-EGFP
- Backbone size w/o insert (bp) 6400
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIL18
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Alt namepro-IL-18
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Alt namePro-Interleukin-18
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SpeciesH. sapiens (human)
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Entrez GeneIL18 (a.k.a. IGIF, IL-18, IL-1g, IL1F4)
- Promoter MESV
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Tags
/ Fusion Proteins
- Myc (N terminal on insert)
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer MSCV-fwd
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV-human-pro-IL-18 was a gift from Jonathan Kagan (Addgene plasmid # 214317 ; http://n2t.net/addgene:214317 ; RRID:Addgene_214317) -
For your References section:
Structural insights into cytokine cleavage by inflammatory caspase-4. Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC. Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9. Epub 2023 Nov 22. 10.1038/s41586-023-06751-9 PubMed 37993712