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pET28_Caspase-4_R269D
(Plasmid #214313)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214313 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CASP4
  • Alt name
    Caspase-4 enzymatic domain
  • Species
    H. sapiens (human)
  • Mutation
    R269D
  • Entrez Gene
    CASP4 (a.k.a. ICE(rel)II, ICEREL-II, ICH-2, Mih1, Mih1/TX, TX)
  • Promoter T7
  • Tag / Fusion Protein
    • His-TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7-fwd
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28_Caspase-4_R269D was a gift from Jonathan Kagan (Addgene plasmid # 214313 ; http://n2t.net/addgene:214313 ; RRID:Addgene_214313)

  • For your References section:

    Structural insights into cytokine cleavage by inflammatory caspase-4. Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC. Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9. Epub 2023 Nov 22. 10.1038/s41586-023-06751-9 PubMed 37993712
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