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pET21b-KRas/13C-FKBP-MBP
(Plasmid #214303)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214303 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b-C-MBP
  • Backbone manufacturer
    Self-built
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Split-KRas fragment fused with FKBP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    900
  • Mutation
    KRas-Q61L
  • Entrez Gene
    KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
  • Promoter T7
  • Tag / Fusion Protein
    • Maltose-binding protein (MBP) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector is built using pET21b(+) as the orginal vector

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21b-KRas/13C-FKBP-MBP was a gift from Cliff Stains (Addgene plasmid # 214303 ; http://n2t.net/addgene:214303 ; RRID:Addgene_214303)

  • For your References section:

    Standardized Parts for Activation of Small GTPase Signaling in Living Cells. He Y, Faulkner BM, Roberti MA, Bassford DK, Stains C. Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499. 10.1002/anie.202403499 PubMed 39058298
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