pET21b-Rac1/13C-FKBP-MBP
(Plasmid #214295)

• Purpose: Express split-Rac1 fragment fused with FKBP and MBP
• Depositing Lab: Cliff Stains
• Publication: He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499.

Backbone

• Vector backbone: pET21b-C-MBP
• Backbone manufacturer: Self-built
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Split-Rac1 fragment fused with FKBP
• Species: H. sapiens (human)
• Insert Size (bp): 921
• Mutation: Rac1-Q61L
• Entrez Gene: RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
• Promoter: T7
• Tag / Fusion Protein:
  • Maltose-binding protein (MBP) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)

Resource Information

• Supplemental Documents:
  • pET21b-Rac1-13C-FKBP-MBP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector is built using pET21b(+) as the original vector