pMAL-MBP-Rac1-T17N
(Plasmid
#214293)
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PurposeExpress dominant negative Rac1 fused with MBP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214293 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMAL-c5X-His
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Backbone manufacturerGenephore
- Backbone size w/o insert (bp) 5695
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDominant negative Rac1
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SpeciesH. sapiens (human)
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Insert Size (bp)543
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MutationRac1-T17N
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Entrez GeneRAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
- Promoter Tac
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Tag
/ Fusion Protein
- Maltose-binding protein (MBP) (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAL-MBP-Rac1-T17N was a gift from Cliff Stains (Addgene plasmid # 214293 ; http://n2t.net/addgene:214293 ; RRID:Addgene_214293) -
For your References section:
Standardized Parts for Activation of Small GTPase Signaling in Living Cells. He Y, Faulkner BM, Roberti MA, Bassford DK, Stains C. Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499. 10.1002/anie.202403499 PubMed 39058298
