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pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX
(Plasmid #214283)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214283 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES
  • Backbone manufacturer
    Takara Bio
  • Backbone size w/o insert (bp) 6105
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Split-RhoA fragments fused with CIDs and FPs
  • Alt name
    mCerulean-FRB-RhoA/N12
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1104
  • Entrez Gene
    RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCerulean (N terminal on insert 1) (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Split-RhoA fragments fused with CIDs and FPs
  • Alt name
    RhoA/13C(T19N)-FKBP-mVenus-CAAX
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1692
  • Mutation
    RhoA-T19N
  • Entrez Gene
    RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
  • Promoter CMV
  • Tag / Fusion Protein
    • mVenus (on insert 2) (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid will express two separate inserts, which include split-GTPase fragments that are fused with different CIDs and FPs

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX was a gift from Cliff Stains (Addgene plasmid # 214283 ; http://n2t.net/addgene:214283 ; RRID:Addgene_214283)

  • For your References section:

    Standardized Parts for Activation of Small GTPase Signaling in Living Cells. He Y, Faulkner BM, Roberti MA, Bassford DK, Stains C. Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499. 10.1002/anie.202403499 PubMed 39058298
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