pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(T17N)-FKBP-mVenus-CAAX
(Plasmid #214281)

• Purpose: Express split-Rac1 fragments that are fused with CIDs and FPs
• Depositing Lab: Cliff Stains
• Publication: He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499.

Backbone

• Vector backbone: pIRES
• Backbone manufacturer: Takara Bio
• Backbone size w/o insert (bp): 6105
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Split-Rac1 fragments fused with CIDs and FPs
• Alt name: mCerulean-FRB-Rac1/N12
• Species: H. sapiens (human)
• Insert Size (bp): 1098
• Entrez Gene: RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCerulean (N terminal on insert 1) (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: Split-Rac1 fragments fused with CIDs and FPs
• Alt name: Rac1/13C(T17N)-FKBP-mVenus-CAAX
• Species: H. sapiens (human)
• Insert Size (bp): 1699
• Mutation: Rac1-T17N
• Entrez Gene: RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mVenus (on insert 2) (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pIRES-mCerulean-FRB-Rac1-N12-Rac1-13C(T17N)-FKBP-mVenus-CAAX.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid will express two separate inserts, which include split-GTPase fragments that are fused with different CIDs and FPs