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pPBbsr-AcGFP1-ORP9-PH
(Plasmid #214270)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214270 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB
  • Backbone manufacturer
    Dr. Allan Bradley (Wellcome Sanger Institute)
  • Backbone size w/o insert (bp) 6753
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AcGFP1-ORP9(1-111)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1177
  • Entrez Gene
    Osbpl9 (a.k.a. Orp-9, AU015843, MGC30367, MGC38458, 2600011I06Rik)
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBbsr-AcGFP1-ORP9-PH was a gift from Shinya Tsukiji (Addgene plasmid # 214270 ; http://n2t.net/addgene:214270 ; RRID:Addgene_214270)

  • For your References section:

    ORP9-PH domain-based fluorescent reporters for visualizing phosphatidylinositol 4-phosphate dynamics in living cells. Ajiki M, Yoshikawa M, Miyazaki T, Kawasaki A, Aoki K, Nakatsu F, Tsukiji S. RSC Chemical Biology 10.1039/D3CB00232B
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