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pSG086
(Plasmid #214263)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214263 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJN105
  • Backbone size w/o insert (bp) 6055
  • Total vector size (bp) 7943
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hybrid PaFtsH protease PaFtsH2H1-link-32 of Pseudomonas aeruginosa SG17M
  • Alt name
    PaFtsH2 encoded by the transmissible locus of stress tolerance tLST
  • Species
    Pseudomonas aeruginosa SG17M
  • Insert Size (bp)
    1918
  • Mutation
    substitution of 31 amino acids of FtsH2 (F124RRFAEKQGMGGFLSIGKSRAKVFMEKNTGV) by 32 amino acids of FtsH1 (M120RQMQGGGGGRGGPMSFGKSKARLLSEDQVET)
  • GenBank ID
    NZ_JALF00000000.1 Z695_RS45375 and Z695_RS57205
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • 6x His-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)
  • 5′ sequencing primer GCGTCTAGACGACTTGCGAGGAACATTGC
  • 3′ sequencing primer CGCGCTAGCTCAGTGATGATGATGATGATGTGGTGTGGACCCTTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG086 was a gift from Ute Romling (Addgene plasmid # 214263 ; http://n2t.net/addgene:214263 ; RRID:Addgene_214263)

  • For your References section:

    The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone C. Mawla GD, Kamal SM, Cao LY, Purhonen P, Hebert H, Sauer RT, Baker TA, Romling U. J Biol Chem. 2024 Feb;300(2):105622. doi: 10.1016/j.jbc.2023.105622. Epub 2024 Jan 3. 10.1016/j.jbc.2023.105622 PubMed 38176647
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