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Addgene

pSG059
(Plasmid #214262)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214262 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 7290
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tLST accessory element PaFtsH2 protease of Pseudomonas aeruginosa SG17M
  • Alt name
    tLST: transmissible locus of stress tolerance
  • Species
    Pseudomonas aeruginosa SG17M
  • Insert Size (bp)
    1921
  • GenBank ID
    NZ_JALF00000000.1 Z695_RS45375
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • 6x His-tag (C terminal on insert)
    • 6xHis-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)
  • 5′ sequencing primer GCGTCTAGACGACTTGCGAGGAACATTGC
  • 3′ sequencing primer CGCGCTAGCTCAGTGATGATGATGATGATGTGGTGTGGACCCTTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG059 was a gift from Ute Romling (Addgene plasmid # 214262 ; http://n2t.net/addgene:214262 ; RRID:Addgene_214262)

  • For your References section:

    The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone C. Mawla GD, Kamal SM, Cao LY, Purhonen P, Hebert H, Sauer RT, Baker TA, Romling U. J Biol Chem. 2024 Feb;300(2):105622. doi: 10.1016/j.jbc.2023.105622. Epub 2024 Jan 3. 10.1016/j.jbc.2023.105622 PubMed 38176647
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