Skip to main content
Addgene

pSG069
(Plasmid #214260)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214260 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pJN105
  • Backbone size w/o insert (bp) 6055
  • Total vector size (bp) 7924
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    tLST accessory genetic element encoded PaFtsH2 proteinase from Pseudomonas aeruginosa SG17M
  • Species
    Pseudomonas aeruginosa SG17M
  • Insert Size (bp)
    1899
  • GenBank ID
    NZ_JALF00000000.1 Z695_RS45375
  • Promoter araBAD promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CGCGCTAGCTTGCGAGGAACATTGCCCATG
  • 3′ sequencing primer GCGTCTAGATCATGGTGTGGACCCTTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG069 was a gift from Ute Romling (Addgene plasmid # 214260 ; http://n2t.net/addgene:214260 ; RRID:Addgene_214260)

  • For your References section:

    The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone C. Mawla GD, Kamal SM, Cao LY, Purhonen P, Hebert H, Sauer RT, Baker TA, Romling U. J Biol Chem. 2024 Feb;300(2):105622. doi: 10.1016/j.jbc.2023.105622. Epub 2024 Jan 3. 10.1016/j.jbc.2023.105622 PubMed 38176647
Related items: