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Addgene

pSG071
(Plasmid #214257)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214257 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJN105
  • Backbone size w/o insert (bp) 6055
  • Total vector size (bp) 7972
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    transfer into Pseudomonas aeruginosa SG17M, SG17M ΔftsH1 ΔftsH2 or other Pseudomonas aeruginosa ΔftsH1 strain
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Hybrid PaFtsH protease PaFtsH1NFtsH2 of Pseudomonas aeruginosa SG17M
  • Species
    Pseudomonas aeruginosa SG17M
  • Insert Size (bp)
    1947
  • Mutation
    Hybrid PaFtsH2NFtsH1 protease: First 18 nucleotides: PaftsH2 Shine-Dalgarno sequence; Hybrid ORF: PaftsH2, nucleotides 1-462 and PaftsH1, nucleotides 454-1920
  • GenBank ID
    NZ_JALF00000000.1 Z695_RS57205 and Z695_RS45375
  • Promoter araBAD promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CGCGCTAGCTTGCGAGGAACATTGCCCATG
  • 3′ sequencing primer GCGTCTAGATCATGGTGTGGACCCTTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG071 was a gift from Ute Romling (Addgene plasmid # 214257 ; http://n2t.net/addgene:214257 ; RRID:Addgene_214257)

  • For your References section:

    The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone C. Mawla GD, Kamal SM, Cao LY, Purhonen P, Hebert H, Sauer RT, Baker TA, Romling U. J Biol Chem. 2024 Feb;300(2):105622. doi: 10.1016/j.jbc.2023.105622. Epub 2024 Jan 3. 10.1016/j.jbc.2023.105622 PubMed 38176647
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