pET28-His6-SUMO-cSrc-SH2-AviTag
(Plasmid #214230)

• Purpose: Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
• Depositing Lab: Neel Shah
• Publication: Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.7554/eLife.82345.

Backbone

• Vector backbone: pET28
• Backbone size w/o insert (bp): 5665
• Total vector size (bp): 5989
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: cSrc kinase SH2 domain
• Alt name: Src-Homology 2 domain of Tyrosine-protein kinase cSrc
• Species: H. sapiens (human)
• Insert Size (bp): 324
• Entrez Gene: SRC (a.k.a. ASV, SRC1, THC6, c-SRC, p60-Src)
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • SUMO (N terminal on backbone)
  • AviTag (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 promotor
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • pET28 HisSUMO Src SH2 Avi.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For efficient biotinylation needs to be expressed in E. coli expression strain containing the BirA expressing plasmid