pAAV-Syn1-DIO-Snca (human A53T)
(Plasmid
#214201)
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PurposeFor Cre-dependent expression of alpha synuclein (human gene containing A53T mutation)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214201 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Total vector size (bp) 4889
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSnca (A53T)
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Alt namealpha syncuclein
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SpeciesH. sapiens (human)
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MutationA53T
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Entrez GeneSNCA (a.k.a. NACP, PARK1, PARK4, PD1)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-Syn1-DIO-Snca (human A53T) was a gift from Thomas Hnasko (Addgene plasmid # 214201 ; http://n2t.net/addgene:214201 ; RRID:Addgene_214201) -
For your References section:
Dopamine neurons exhibit emergent glutamatergic identity in Parkinson's disease. Steinkellner T, Conrad WS, Kovacs I, Rissman RA, Lee EB, Trojanowski JQ, Freyberg Z, Roy S, Luk KC, Lee VM, Hnasko TS. Brain. 2022 Apr 29;145(3):879-886. doi: 10.1093/brain/awab373. 10.1093/brain/awab373 PubMed 35258081