pAAV-Syn1-DIO-VGLUT2:miniSOG
(Plasmid #214200)

• Purpose: For Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)
• Depositing Lab: Thomas Hnasko
• Publication: Steinkellner et al ACS Chem Neurosci. 2021 Feb 17;12(4):626-639. doi: 10.1021/acschemneuro.0c00643. Epub 2021 Feb 1.

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Backbone

• Vector backbone: pAAV DIO
• Total vector size (bp): 6962
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Slc17a6 tagged with miniSOG and hemagglutinin
• Alt name: VGLUT2
• Alt name: miniSOG
• Tag / Fusion Protein:
  • miniSOG (tagged with HA) is fused to the first intralumenal loop of rat Slc17a6 (VGLUT2)

Resource Information

• Supplemental Documents:
  • 308a_AAV-hSyn1-DIO-VGLUT2.miniSOG_[cre_on].gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry