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Addgene

pAAV-Syn1-DIO-VGLUT2:miniSOG
(Plasmid #214200)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214200 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV DIO
  • Total vector size (bp) 6962
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Slc17a6 tagged with miniSOG and hemagglutinin
  • Alt name
    VGLUT2
  • Alt name
    miniSOG
  • Tag / Fusion Protein
    • miniSOG (tagged with HA) is fused to the first intralumenal loop of rat Slc17a6 (VGLUT2)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Syn1-DIO-VGLUT2:miniSOG was a gift from Thomas Hnasko (Addgene plasmid # 214200 ; http://n2t.net/addgene:214200 ; RRID:Addgene_214200)

  • For your References section:

    Genetic Probe for Visualizing Glutamatergic Synapses and Vesicles by 3D Electron Microscopy. Steinkellner T, Madany M, Haberl MG, Zell V, Li C, Hu J, Mackey M, Ramachandra R, Adams S, Ellisman MH, Hnasko TS, Boassa D. ACS Chem Neurosci. 2021 Feb 17;12(4):626-639. doi: 10.1021/acschemneuro.0c00643. Epub 2021 Feb 1. 10.1021/acschemneuro.0c00643 PubMed 33522227
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