pCrAb-km
(Plasmid
#214199)
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PurposeExpresses crRNA for genome editing in Acinetobacter baumannii
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214199 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC57 and pWH1266
- Total vector size (bp) 6110
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecrRNA-HDV
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SpeciesSynthetic
- Promoter J23119
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer tgggcataaagttgcaagcttGACAGCTAGCTCAGTCCTAGGTATAATAC
- 3′ sequencing primer tcccccgggctgcaggaattcGGTAGACCAGAAACAAAAAAACACC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCrAb-km was a gift from Yu Wang (Addgene plasmid # 214199 ; http://n2t.net/addgene:214199 ; RRID:Addgene_214199) -
For your References section:
The Development of a CRISPR-FnCpf1 System for Large-Fragment Deletion and Multiplex Gene Editing in Acinetobacter baumannii. Wang S, Ding Y, Rong H, Wang Y. Curr Issues Mol Biol. 2024 Jan 5;46(1):570-584. doi: 10.3390/cimb46010037. 10.3390/cimb46010037 PubMed 38248339
