pFnCpfAb-apr
(Plasmid
#214198)
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PurposeExpresses FnCpf1 and RecAb recombination system for genome editing in Acinetobacter baumannii
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214198 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMMB67EH
- Total vector size (bp) 16376
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameFnCpf1
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Alt nameFnCas12a
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SpeciesFrancisella novicida U112
- Promoter tac promoter
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer tgagacaataaccctggatccGGGCTTATCGACTGCAC
- 3′ sequencing primer agctgcacatgaaccggatccGTGAATTGACGCGTATTGGGATGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFnCpfAb-apr was a gift from Yu Wang (Addgene plasmid # 214198 ; http://n2t.net/addgene:214198 ; RRID:Addgene_214198) -
For your References section:
The Development of a CRISPR-FnCpf1 System for Large-Fragment Deletion and Multiplex Gene Editing in Acinetobacter baumannii. Wang S, Ding Y, Rong H, Wang Y. Curr Issues Mol Biol. 2024 Jan 5;46(1):570-584. doi: 10.3390/cimb46010037. 10.3390/cimb46010037 PubMed 38248339