pcDNA3.1-mGCC2-FLAG
(Plasmid
#214166)
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PurposeTransient expression of GCC2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214166 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCC2
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SpeciesM. musculus (mouse)
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Entrez GeneGcc2 (a.k.a. 0610043A03Rik, 2210420P05Rik, 2600014C01Rik, AW112121, mKIAA0336)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV_fwd
- 3′ sequencing primer BGH_rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-mGCC2-FLAG was a gift from Nan Yan (Addgene plasmid # 214166 ; http://n2t.net/addgene:214166 ; RRID:Addgene_214166) -
For your References section:
Interruption of post-Golgi STING trafficking activates tonic interferon signaling. Tu X, Chu TT, Jeltema D, Abbott K, Yang K, Xing C, Han J, Dobbs N, Yan N. Nat Commun. 2022 Nov 15;13(1):6977. doi: 10.1038/s41467-022-33765-0. 10.1038/s41467-022-33765-0 PubMed 36379959