pMRX-hSTING (R232)
(Plasmid
#214162)
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PurposeRetroviral transduction of STING
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214162 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMRX
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Vector typeRetroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSTING
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SpeciesH. sapiens (human)
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Entrez GeneSTING1 (a.k.a. ERIS, MITA, MPYS, NET23, SAVI, STING, STING-beta, TMEM173, hMITA, hSTING)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TGGACCATCCTCTAGACTG
- 3′ sequencing primer CGATACTTGGCGTAATCAT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRX-hSTING (R232) was a gift from Nan Yan (Addgene plasmid # 214162 ; http://n2t.net/addgene:214162 ; RRID:Addgene_214162) -
For your References section:
Interferon-Independent Activities of Mammalian STING Mediate Antiviral Response and Tumor Immune Evasion. Wu J, Dobbs N, Yang K, Yan N. Immunity. 2020 Jul 14;53(1):115-126.e5. doi: 10.1016/j.immuni.2020.06.009. Epub 2020 Jul 7. 10.1016/j.immuni.2020.06.009 PubMed 32640258